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The design of an all-reflective laser-scanning microscope capable of imaging samples three-dimensionally with multiple modes of nonlinear imaging at high resolution and with a large field of view is described in this presentation. The all-reflective design is based on off-axis parabolic mirror sections, and was designed to require minimal alignment. An all fiber alignment-free femtosecond laser with dual output is used to enable simultaneous multiphoton and stimulated Raman imaging processes, creating a powerful but hassle-free system. This paper will describe the design, performance, and imaging outputs of the system.
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