The design of an all-reflective laser-scanning microscope capable of imaging samples three-dimensionally with multiple modes of nonlinear imaging at high resolution and with a large field of view is described in this presentation. The all-reflective design is based on off-axis parabolic mirror sections, and was designed to require minimal alignment. An all fiber alignment-free femtosecond laser with dual output is used to enable simultaneous multiphoton and stimulated Raman imaging processes, creating a powerful but hassle-free system. This paper will describe the design, performance, and imaging outputs of the system.
Raman microscopy is a key technique for biological imaging since it can provide valuable information about the chemical constituents of a sample without any labels. However, because two wavelengths are required for either CARS or SRS to occur, most Raman imaging set ups use multiple lasers with complicated synchronization requirements. In this presentation, we discuss the design and performance of a tunable Ytterbium-based fiber laser and an optical parametric oscillator for Raman microscopy. Our system uses a single laser that creates both pump and probe beams via nonlinear optical effects. Due to its reasonable high peak power, this laser system is a suitable light source for multimodal microscopy using both Raman and multiphoton imaging functionalities.
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