Brillouin and Raman imaging for plant cell wall mechanics

Timm Landes; Inga Weisheit; Alphonse Mathew; Eren Alkanat; Miroslav Zabic; Thomas Debener; Dag Heinemann

doi:10.1117/12.2649829

16 March 2023 Brillouin and Raman imaging for plant cell wall mechanics

Timm Landes, Inga Weisheit, Alphonse Mathew, Eren Alkanat, Miroslav Zabic, Thomas Debener, Dag Heinemann

Proceedings Volume 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; 123910K (2023) https://doi.org/10.1117/12.2649829
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Plant cell walls are highly complex structures, which are adaptable to various environmental stresses. While many biochemical pathways have been discovered, cellular stress indicators are widely unknown. Here we demonstrate a label-free optical setup, that can map both mechanical properties and chemical composition in biological samples. We measured changes in mechanical stiffness and chemical response of rose leaf cells due to inoculation of the fungal disease Diplocarpon rosae. With this we are able to image fungal growth in a dynamical and high-resolution manner.

Conference Presentation

Citation Download Citation

Timm Landes, Inga Weisheit, Alphonse Mathew, Eren Alkanat, Miroslav Zabic, Thomas Debener, and Dag Heinemann "Brillouin and Raman imaging for plant cell wall mechanics", Proc. SPIE 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, 123910K (16 March 2023); https://doi.org/10.1117/12.2649829

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