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13 March 2024 Multiplexed fibre-optic real-time monitoring of fluorophores in perfused hearts
Jianrong Qiu, Edward Waters, Friedrich Baark, Elzbieta Stepula, Antoine L. D. Wallabregue, Stuart J. Conway, Richard Southworth, Mads S. Bergholt
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Proceedings Volume 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024; 128190H (2024) https://doi.org/10.1117/12.3002462
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We have developed a prototype multiplexed fiber-optic system that enables real-time monitoring of hypoxia-sensing fluorophores in beating isolated rat hearts, providing dynamic data useful for the assessment of cardiac metabolism, with potential for cross-correlation and biological validation using parallel MR spectroscopy and PET or SPECT imaging. The system has been designed to suppress background and increase light efficiency. We have established a data analysis pipeline using nonnegative least-squares curve fitting allowing unmixing of overlapped fluorescence spectra and fluorophore quantification. In this first phase of our work, we demonstrate simultaneous detection of a mixture of fluorophores in isolated perfused hearts.
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Jianrong Qiu, Edward Waters, Friedrich Baark, Elzbieta Stepula, Antoine L. D. Wallabregue, Stuart J. Conway, Richard Southworth, and Mads S. Bergholt "Multiplexed fibre-optic real-time monitoring of fluorophores in perfused hearts", Proc. SPIE 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024, 128190H (13 March 2024); https://doi.org/10.1117/12.3002462
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KEYWORDS
Fluorophores
Heart
Multiplexing
Fluorescence
Imaging spectroscopy
Spectroscopy
Magnetic resonance imaging
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