High-sensitivity confocal imaging with bilateral scanning and CCD detectors

G. J. Brakenhoff; Koen Visscher

doi:10.1117/12.59582

26 June 1992 High-sensitivity confocal imaging with bilateral scanning and CCD detectors

G. J. Brakenhoff, Koen Visscher

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59582
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

The high quantum efficiency and high red sensitivity of a charge coupled device (CCD) make it a very suitable detector for confocal fluorescence imaging, especially for applications in biology, where short wavelength illumination may cause undesirable radiation damage. In addition, its high dynamic range matches the high contrast imaging inherent in confocal microscopy, and enables one to record small intensity differences if this dynamic range is well exploited. Applications shown include imaging with bleaching rate and non-linear fluorescence excitation as parameters. The bilateral scan technique permits effective confocal imaging using this type of device. CCDs can be used for confocal imaging both in high speed (up to real- time) applications as well as in the integration mode, where the high sensitivity application areas for this confocal technique are present.

Citation Download Citation

G. J. Brakenhoff and Koen Visscher "High-sensitivity confocal imaging with bilateral scanning and CCD detectors", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59582

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