Laser-mediated variations in merocyanine 540 incorporation into phospholipid membranes

Libusa Sikurova; R. Frankova; Jan Psenica; Dusan Chorvat

doi:10.1117/12.146193

17 June 1993 Laser-mediated variations in merocyanine 540 incorporation into phospholipid membranes

Libusa Sikurova, R. Frankova, Jan Psenica, Dusan Chorvat

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146193
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The influence of Nd/YAG laser irradiation (532 nm) on the MC-540 stained POPC and DMPC liposomes (lipid:dye ratio 42:1 - 20:1) was studied by absorption spectroscopy in the range of 380 - 700 nm. Two absorption bands (533 nm and 567 nm) corresponding to two colored species of the dye -- monomers and dimers -- were observed for MC-540 in phospholipid liposomes. MC-40 monomer-dimer distribution was shown to depend on the liposome composition and lipid:dye ratio. Irradiation of the samples by laser light of fluence rate 3000 W/m² resulted only in MC-540 monomer-dimer distribution changes. The variation of this effect was observed as the kind of liposomes (alcoholic or ultrasonic) were changed. MC-540 monomer-dimer distribution is one of the factors that affect the dye- mediated photoinactivation. Illumination by intensive laser light (60,000 W/m²) caused color fading of the MC-540 dye.

Citation Download Citation

Libusa Sikurova, R. Frankova, Jan Psenica, and Dusan Chorvat "Laser-mediated variations in merocyanine 540 incorporation into phospholipid membranes", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146193

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