Optical activity measurement of multicomponent biological substances

Vasyl V. Molebny; Valeri V. Marienko

doi:10.1117/12.167324

1 February 1994 Optical activity measurement of multicomponent biological substances

Vasyl V. Molebny, Valeri V. Marienko

Proceedings Volume 2084, Biomedical Optoelectronic Devices and Systems; (1994) https://doi.org/10.1117/12.167324
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Information on optical activity of biological substances under test is often influenced by other ingredients with optical activity or fenomena interfering with the process of polarization rotation. The influence of cell-medium interface can be excluded by solving the two-equation system, following from two independent measurements of optical activity of the same biological substance in two identical cells of different lengths. In a similar way, the system of n equations can be found by excluding the interfering components, supposing their frequency- dispersion or temperature dependences are known from previously made experiments. Our measuring system uses polarization modulation (linear rotation by Faraday modulator). For 10% depolarization in solutions, we achieved an accuracy of 15 arc. sec at 2 mW laser power and simple film analyzers are enough for such results. A microprocessor simplifies the routine calculations.

Citation Download Citation

Vasyl V. Molebny and Valeri V. Marienko "Optical activity measurement of multicomponent biological substances", Proc. SPIE 2084, Biomedical Optoelectronic Devices and Systems, (1 February 1994); https://doi.org/10.1117/12.167324

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