Volume changes of human endothelial cells induced by photodynamic treatment

Andreas Leunig; Frank Staub; Nick Plesnila; Jurgen Peters; Jens Feyh; Ralph Gutmann; Alwin E. Goetz

doi:10.1117/12.230963

31 January 1996 Volume changes of human endothelial cells induced by photodynamic treatment

Andreas Leunig, Frank Staub, Nick Plesnila, Jurgen Peters, Jens Feyh, Ralph Gutmann, Alwin E. Goetz

Proceedings Volume 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities; (1996) https://doi.org/10.1117/12.230963
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Photodynamic therapy (PDT) has shown promising results in treatment of malignant tumors. However, the mechanisms leading to tumor destruction during PDT are still not completely understood. In addition to effects on the microcirculation, damage to cellular structures has been observed following exposure of cells to PDT. A phenomenon preceding these events might possibly be cell swelling. We therefore studied the influence of treatment with Photofrin (PF) and laser light on volume changes and cell viability of endothelial cells. Endothelial cells were obtained from human umbilical cord veins (HUVEC) by an adaption of the method of Maruyama (1963). After subcultivation the cells were harvested and transferred as a cell suspension into a specially designed incubation chamber. Cells received either PF in concentrations of 1.5 or 3.0 (mu) g/ml and laser illumination (630 nm; 40 mW/cm², 4 Joule), PF alone, or laser treatment only. Following start of PF incubation and after phototreatment cell samples were taken for volume measurements using flow cytometry and for studies of cellular morphology using scanning electron microscopy. Simultaneously, cell viability was monitored by the trypan blue exclusion test and colorimetric MTT assay. (abstract truncated)

Citation Download Citation

Andreas Leunig, Frank Staub, Nick Plesnila, Jurgen Peters, Jens Feyh, Ralph Gutmann, and Alwin E. Goetz "Volume changes of human endothelial cells induced by photodynamic treatment", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.230963

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