In vivo characterization of cyanine dyes as contrast agents for near-infrared imaging

Bjoern Riefke; Kai Licha; Wolfhard Semmler; Dirk Nolte; Bernd Ebert; Herbert H. Rinneberg

doi:10.1117/12.260652

11 December 1996 In vivo characterization of cyanine dyes as contrast agents for near-infrared imaging

Bjoern Riefke, Kai Licha, Wolfhard Semmler, Dirk Nolte, Bernd Ebert, Herbert H. Rinneberg

Proceedings Volume 2927, Optical and Imaging Techniques for Biomonitoring II; (1996) https://doi.org/10.1117/12.260652
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

In this study indotricarbocyanines were investigated in vivo as near-infrared contrast agents. The known dye indocyanine green (ICG) has several disadvantages regarding its use in near-infrared imaging. ICG has a very short plasma half- life, limited tolerability and is unstable in aqueous solutions. Therefore, several indotricarbocyanine dyes, structurally related to ICG but with different hydrophilicities and physicochemical properties, were synthesized. The tolerability of synthesized dyes was tested in mice. The pharmacokinetic behavior and elimination characteristics were studied in a rat model. The in vivo imaging properties of synthesized dyes were investigated using a tunable, pulsed, solid state laser system for excitation and an intensified CCD camera for fluorescence imaging of different tumor-bearing nude mice models and mamma-carcinoma-bearing rat models. The dye-specific fluorescence exitance was followed at different times after dye administration. The results are demonstrated in comparison to indocyanine green. Synthesized hydrophilic indotricarbocyanine dyes had longer plasma half-lives and increasing renal elimination, corresponding to higher hydrophilicity. Tolerability in mice was increased up to 60- fold compared to ICG. Increased fluorescence exitance in tumors was observed for several dyes 24 h p.i. in the tumor models studied, whereas ICG showed no tumor fluorescence signal under the same conditions.

Citation Download Citation

Bjoern Riefke, Kai Licha, Wolfhard Semmler, Dirk Nolte, Bernd Ebert, and Herbert H. Rinneberg "In vivo characterization of cyanine dyes as contrast agents for near-infrared imaging", Proc. SPIE 2927, Optical and Imaging Techniques for Biomonitoring II, (11 December 1996); https://doi.org/10.1117/12.260652

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