Luminescent probes for optical in vivo imaging

Isabelle Texier; Veronique Josserand; Elisabeth Garanger; Jesus Razkin; Zhaohui Jin; Pascal Dumy; Marie Favrot; Didier Boturyn; Jean-Luc Coll

doi:10.1117/12.588162

4 April 2005 Luminescent probes for optical in vivo imaging

Isabelle Texier, Veronique Josserand, Elisabeth Garanger, Jesus Razkin, Zhaohui Jin, Pascal Dumy, Marie Favrot, Didier Boturyn, Jean-Luc Coll

Author Affiliations +

Proceedings Volume 5704, Genetically Engineered and Optical Probes for Biomedical Applications III; (2005) https://doi.org/10.1117/12.588162
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Going along with instrumental development for small animal fluorescence in vivo imaging, we are developing molecular fluorescent probes, especially for tumor targeting. Several criteria have to be taken into account for the optimization of the luminescent label. It should be adapted to the in vivo imaging optical conditions : red-shifted absorption and emission, limited overlap between absorption and emission for a good signal filtering, optimized luminescence quantum yield, limited photo-bleaching. Moreover, the whole probe should fulfill the biological requirements for in vivo labeling : adapted blood-time circulation, biological conditions compatibility, low toxicity. We here demonstrate the ability of the imaging fluorescence set-up developed in LETI to image the bio-distribution of molecular probes on short times after injection. Targeting with Cy5 labeled holo-transferrin of subcutaneous TS/Apc (angiogenic murine breast carcinoma model) or IGROV1 (human ovarian cancer) tumors was achieved. Differences in the kinetics of the protein uptake by the tumors were evidenced. IGROV1 internal metastatic nodes implanted in the peritoneal cavity could be detected in nude mice. However, targeted metastatic nodes in lung cancer could only be imaged after dissection of the mouse. These results validate our fluorescence imaging set-up and the use of Cy5 as a luminescent label. New fluorescent probes based on this dye and a molecular delivery template (the RAFT molecule) can thus be envisioned.

Citation Download Citation

Isabelle Texier, Veronique Josserand, Elisabeth Garanger, Jesus Razkin, Zhaohui Jin, Pascal Dumy, Marie Favrot, Didier Boturyn, and Jean-Luc Coll "Luminescent probes for optical in vivo imaging", Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); https://doi.org/10.1117/12.588162

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available