Optoacoustics, laser-induced fluorescence (LIF), and photometry for investigation of different skin types in vitro and in vivo

Myriam Bartels; Merve Meinhardt; Ronald Krebs; Holger Petering; Thomas Werfel; Angelika Anders

doi:10.1117/12.500625

14 October 2003 Optoacoustics, laser-induced fluorescence (LIF), and photometry for investigation of different skin types in vitro and in vivo

Myriam Bartels, Merve Meinhardt, Ronald Krebs, Holger Petering, Thomas Werfel, Angelika Anders

Author Affiliations +

Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500625
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Precise determination as well as comparison of optical properties of human skin in vivo and in vitro is of great importance to the understanding of effects of UV exposure. Because of that, the absorption properties of epidermal models without and with elanocytes of skin type IV and VI were examined using optical and optoacoustic spectroscopy. The effect of melanin as an important chromophor in human skin was investigated using a photometer, laser induced fluorescence (LIF) and optoacoustics. Moreover, an epidemal model irradiated several times with UVA showed similar absorption characteristics as human skin in vivo. Besides, optoacoustic signals are shown to deliver structural characteristics of different epidermal layers that are about 40 μm thick. Using laser optoacoustics and laser induced fluorescence, human skin in vivo can be investigated wavelength-resolved. Therefore, optoacoustics is a promising tool for in vivo determination of different skin types, optimization of phototherapy and testing of protective substances like sunscreens in the future.

Citation Download Citation

Myriam Bartels, Merve Meinhardt, Ronald Krebs, Holger Petering, Thomas Werfel, and Angelika Anders "Optoacoustics, laser-induced fluorescence (LIF), and photometry for investigation of different skin types in vitro and in vivo", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500625

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Absorption

In vivo imaging

Laser induced fluorescence

Luminescence

Ultraviolet radiation

In vitro testing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years