Measurement of retinal blood velocity

Leonard W. Winchester; Nee-Yin Chou

doi:10.1117/12.646683

7 March 2006 Measurement of retinal blood velocity

Leonard W. Winchester, Nee-Yin Chou

Proceedings Volume 6138, Ophthalmic Technologies XVI; 61381N (2006) https://doi.org/10.1117/12.646683
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A fundus camera was modified to illuminate the retina of a rabbit model with low power laser light in order to obtain laser speckle images. A fast-exposure charge-coupled device (CCD) camera was used to capture laser speckle images of the retina. Image acquisition was synchronized with the arterial pulses of the rabbit to ensure that all images are obtained at the same point in the cardiac cycle. The rabbits were sedated and a speculum was inserted to prevent the eyelid from closing. Both albino (New Zealand) and pigmented (Dutch belted) rabbits were used in the study. The rabbit retina is almost avascular. The measurements are obtained for choroidal tissue as well as retinal tissue. Because the retina is in a region of high metabolism, blood velocity is strongly affected by blood oxygen saturation. Measurements of blood velocity obtained over a wide range of O₂ saturations (58%-100%) showed that blood velocity increases with decreasing O₂ saturation. For most experiments, the left eye of the rabbit was used for laser measurements whereas the right eye served as a control. No observable difference between pre- and post-experimented eye was noted. Histological examinations of retinal tissue subjected to repeated laser measurements showed no indication of tissue damage.

Citation Download Citation

Leonard W. Winchester and Nee-Yin Chou "Measurement of retinal blood velocity", Proc. SPIE 6138, Ophthalmic Technologies XVI, 61381N (7 March 2006); https://doi.org/10.1117/12.646683

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available