A fluorescence sectioning endoscopy using dynamic speckle illumination

Jun Yin; Yonghong Shao; Junle Qu D.D.S.; Haoming Lin; Hanben Niu

doi:10.1117/12.764304

21 February 2008 A fluorescence sectioning endoscopy using dynamic speckle illumination

Jun Yin, Yonghong Shao, Junle Qu D.D.S., Haoming Lin, Hanben Niu

Proceedings Volume 6851, Endoscopic Microscopy III; 68510N (2008) https://doi.org/10.1117/12.764304
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We present a novel fluorescence sectioning endoscopy that is based on a fiber-bundle and using speckle pattern illumination. The laser speckle pattern is produced by a rotating diffuser and delivered into the fiber bundle for whole-field excitation of the sample. The fluorescence is collected and transmitted to a CCD camera via the endoscope optics and fiber bundle. A sequence of fluorescent images are acquired and processed to reconstruct a 2D depth-resolved image of the sample. The fiber-bundle based endoscopy has similar sectioning capability to that of a laser scanning confocal microscopy but without scanning. Its other advantages include compactness and low cost, which makes it potentially viable for implementation in a portable clinical system.

Citation Download Citation

Jun Yin, Yonghong Shao, Junle Qu D.D.S., Haoming Lin, and Hanben Niu "A fluorescence sectioning endoscopy using dynamic speckle illumination", Proc. SPIE 6851, Endoscopic Microscopy III, 68510N (21 February 2008); https://doi.org/10.1117/12.764304

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available