Rapid detection of bacterial cells by light scattering method

Oleksandr I. Bilyy; Vasyl' B. Getman; Rostyslav O. Bilyy; Igor M. Kushnir; Igor Ya. Kotsyumbas

doi:10.1117/12.762744

1 March 2008 Rapid detection of bacterial cells by light scattering method

Oleksandr I. Bilyy, Vasyl' B. Getman, Rostyslav O. Bilyy, Igor M. Kushnir, Igor Ya. Kotsyumbas

Proceedings Volume 6864, Biomedical Applications of Light Scattering II; 686411 (2008) https://doi.org/10.1117/12.762744
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Method of rapid detection of bacterial cells by light scattering is described. Determination of quantitative changes of bacteria is the given method based on the changes of their size distributing in the process of cultivation. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. A method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses. Distribution of particles by sizes is determined from the measured functional dependence of number of registered particles from amplitude and duration of the proper electric impulses on the output photoreceiver. Detection is done for a range of particle size from 0.1 to 10 mkm, and thus particle's size distribution is determined. The results of studying of rapid detection of Escherichia coli by light scattering are described.

Citation Download Citation

Oleksandr I. Bilyy, Vasyl' B. Getman, Rostyslav O. Bilyy, Igor M. Kushnir, and Igor Ya. Kotsyumbas "Rapid detection of bacterial cells by light scattering method", Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 686411 (1 March 2008); https://doi.org/10.1117/12.762744

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