Enhancing surface plasmon detection of biomolecular interactions through use of nanostructured interfaces

S. Krishnamoorthy; M. Himmelhaus

doi:10.1117/12.763118

21 February 2008 Enhancing surface plasmon detection of biomolecular interactions through use of nanostructured interfaces

S. Krishnamoorthy, M. Himmelhaus

Author Affiliations +

Proceedings Volume 6869, Plasmonics in Biology and Medicine V; 68690F (2008) https://doi.org/10.1117/12.763118
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In this work, we aim at enhancing the sensitivity of surface plasmon resonance sensors towards the detection of biomolecule interactions by means of nanopatterning of the sensor surface. Use of nanostructured interfaces in combination with SPR is a promising step towards realizing biosensors with high efficiency and sensitivity. Nanopatterned surfaces enable multi-dimensional control over the behavior of surface-immobilized probe molecules. By means of a combination of self-assembled monolayer technology, colloidal lithography, and reactive ion etching, nanopatterns with either antibody confining or non-confining characteristics were produced and analyzed via photoelectron spectroscopy and infrared reflection absorption spectroscopy. Antibody immobilization on the patterns and subsequent specific binding of antigen was traced in real time by means of a surface plasmon resonance sensor. It was found that confining nanopatterns yield an increase in antibody activity towards antigen capture on surface of up to 120%, depending on the protocol used for their immobilization.

Citation Download Citation

S. Krishnamoorthy and M. Himmelhaus "Enhancing surface plasmon detection of biomolecular interactions through use of nanostructured interfaces", Proc. SPIE 6869, Plasmonics in Biology and Medicine V, 68690F (21 February 2008); https://doi.org/10.1117/12.763118

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