Peptide linkers for the assembly of semiconductor quantum dot bioconjugates

Kelly Boeneman; Bing C. Mei; Jeffrey R. Deschamps; James B. Delehanty; Hedi Mattoussi; Igor Medintz

doi:10.1117/12.808540

3 March 2009 Peptide linkers for the assembly of semiconductor quantum dot bioconjugates

Kelly Boeneman, Bing C. Mei, Jeffrey R. Deschamps, James B. Delehanty, Hedi Mattoussi, Igor Medintz

Proceedings Volume 7189, Colloidal Quantum Dots for Biomedical Applications IV; 71890G (2009) https://doi.org/10.1117/12.808540
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The use of semiconductor luminescent quantum dots for the labeling of biomolecules is rapidly expanding, however it still requires facile methods to attach functional globular proteins to biologically optimized quantum dots. Here we discuss the development of controlled variable length peptidyl linkers to attach biomolecules to poly(ethylene) glycol (PEG) coated quantum dots for both in vitro and in vivo applications. The peptides chosen, β-sheets and alpha helices are appended to polyhistidine sequences and this allows for control of the ratio of peptide bioconjugated to QD and the distance from QD to the biomolecule. Recombinant DNA engineering, bacterial peptide expression and Ni-NTA purification of histidine labeled peptides are utilized to create the linkers. Peptide length is confirmed by in vitro fluorescent resonance energy transfer (FRET).

Citation Download Citation

Kelly Boeneman, Bing C. Mei, Jeffrey R. Deschamps, James B. Delehanty, Hedi Mattoussi, and Igor Medintz "Peptide linkers for the assembly of semiconductor quantum dot bioconjugates", Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 71890G (3 March 2009); https://doi.org/10.1117/12.808540

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