Three-dimensional multimodal microscopy of rabbit cornea after cross-linking treatment

A. Krüger; M. Hovakimyan; D. F. Ramírez; R.-A. Lorbeer; M. Kröger; O. Stachs; A. Wree; R. F. Guthoff; H. Lubatschowski; A. Heisterkamp

doi:10.1117/12.842282

2 March 2010 Three-dimensional multimodal microscopy of rabbit cornea after cross-linking treatment

A. Krüger, M. Hovakimyan, D. F. Ramírez, R.-A. Lorbeer, M. Kröger, O. Stachs, A. Wree, R. F. Guthoff, H. Lubatschowski, A. Heisterkamp

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Proceedings Volume 7550, Ophthalmic Technologies XX; 755002 (2010) https://doi.org/10.1117/12.842282
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.

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A. Krüger, M. Hovakimyan, D. F. Ramírez, R.-A. Lorbeer, M. Kröger, O. Stachs, A. Wree, R. F. Guthoff, H. Lubatschowski, and A. Heisterkamp "Three-dimensional multimodal microscopy of rabbit cornea after cross-linking treatment", Proc. SPIE 7550, Ophthalmic Technologies XX, 755002 (2 March 2010); https://doi.org/10.1117/12.842282

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KEYWORDS

Second-harmonic generation

Cornea

Confocal microscopy

Microscopy

Reflectivity

Collagen

Femtosecond phenomena

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