Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells

Wei-wei Zhang; Zhi-ping Wang; Tong-sheng Chen

doi:10.1117/12.839631

11 February 2010 Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells

Wei-wei Zhang, Zhi-ping Wang, Tong-sheng Chen

Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650K (2010) https://doi.org/10.1117/12.839631
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Resveratrol (RV) is a natural plant polyphenol widely present in foods such as grapes, wine, and peanuts. Previous studies indicate that RV has an ability to inhibit various stages of carcinogenesis and eliminate preneoplastic cells in vitro and in vivo. However, little is known about the molecular mechanism of RV-induced apoptosis in human lung adenocarcinoma (ASTC-a-1) cell. In this report, we analyzed whether Bax translocation from cytoplasm to mitochondria during RV-induced apoptosis in single living cell using onfocal microscopey. Cells were transfected with GFP-Bax plasmid. Cell counting kit (CCK-8) assay was used to assess the inhibition of RV on the cells viability. Apoptotic activity of RV was detected by Hoechst 33258 and propidium iodide (PI) staining. Our results showed that RV induced a dose-dependent apoptosis in which Bax did not translocate to mitochondrias.

Citation Download Citation

Wei-wei Zhang, Zhi-ping Wang, and Tong-sheng Chen "Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650K (11 February 2010); https://doi.org/10.1117/12.839631

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