Differential interference contrast microscopy for the quantitative assessment of tissue organization

Donald Duncan; David Fischer; Mehran Daneshbod; Amanda Dayton; Scott Prahl

doi:10.1117/12.842802

24 February 2010 Differential interference contrast microscopy for the quantitative assessment of tissue organization

Donald Duncan, David Fischer, Mehran Daneshbod, Amanda Dayton, Scott Prahl

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700C (2010) https://doi.org/10.1117/12.842802
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The propagation of light through complex structures, such as biological tissue, is a poorly understood phenomenon. Typically the tissue is modeled as an effective medium, and Monte Carlo techniques are used to solve the radiative transport equation. In such an approach the medium is characterized in terms of a limited number of physical scatter and absorption parameters, but is otherwise considered homogeneous. For exploration of propagation phenomena such as spatial coherence, however, a physical model of the tissue medium that allows multiscale structure is required. We present a particularly simple means of establishing such a multiscale tissue characterization based on measurements using a differential interference contrast (DIC) microscope. This characterization is in terms of spatially resolved maps of the (polar and azimuthal) angular ray deviations. With such data, tissues can be characterized in terms of their first and second order scatter properties. We discuss a simple means of calibrating a DIC microscope, the measurement procedure and quantitative interpretation of the ensuing data. These characterizations are in terms of the scatter phase function and the spatial power spectral density

Citation Download Citation

Donald Duncan, David Fischer, Mehran Daneshbod, Amanda Dayton, and Scott Prahl "Differential interference contrast microscopy for the quantitative assessment of tissue organization", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700C (24 February 2010); https://doi.org/10.1117/12.842802

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