Cell cycle imaging with quantitative differential interference contrast microscopy

Piotr Kostyk; Shelley Phelan; Min Xu

doi:10.1117/12.2005540

22 February 2013 Cell cycle imaging with quantitative differential interference contrast microscopy

Piotr Kostyk, Shelley Phelan, Min Xu

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85870J (2013) https://doi.org/10.1117/12.2005540
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We report a microscopic approach for determining cell cycle stages by measuring the nuclear optical path length (OPL) with quantitative differential interference contrast (DIC) microscopy. The approach is validated by the excellent agreement between the proportion of proliferating-to-quiescent cancerous breast epithelial cells obtained from DIC microscopy, and that from a standard immunofluorescence assay.

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Piotr Kostyk, Shelley Phelan, and Min Xu "Cell cycle imaging with quantitative differential interference contrast microscopy", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85870J (22 February 2013); https://doi.org/10.1117/12.2005540

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KEYWORDS

Digital image correlation

Microscopy

Refractive index

Cancer

Tissue optics

Proteins

Tissues

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