Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation

Honghai Liu; Wan Qin; Yonghong Shao; Zhonghai Wang; Huaxiao Yang; Raymond B. Runyan; Thomas K. Borg; Bruce Gao

doi:10.1117/12.2005156

26 February 2013 Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation

Honghai Liu, Wan Qin, Yonghong Shao, Zhonghai Wang, Huaxiao Yang, Raymond B. Runyan, Thomas K. Borg, Bruce Gao

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871W (2013) https://doi.org/10.1117/12.2005156
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Using hybrid TPEF-SHG imaging and immunocytological techniques, we studied dedifferentiation of adult cardiomyocytes. First, the myofibrils shrank to shorten the sarcomere length. At the cell ends, the striated pattern of myosin filaments began to dissociate; at the center of the cell, the striated pattern of alpha-actinin first faded away and reappeared near the cell membrane during dedifferentiation. The results suggest that when freshly isolated adult cardiomyocytes are used to model cardiac muscle, the end-to-end connection may be important to maintain their striated myofibrillar structure and rod-shape morphology.

Citation Download Citation

Honghai Liu, Wan Qin, Yonghong Shao, Zhonghai Wang, Huaxiao Yang, Raymond B. Runyan, Thomas K. Borg, and Bruce Gao "Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871W (26 February 2013); https://doi.org/10.1117/12.2005156

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