Dr. Zhonghai Wang Profile

Dr. Zhonghai Wang

at Clemson Univ

SPIE Involvement:

Author

Publications (4)

Proceedings Article | 14 February 2020 Paper

Study of the sarcomeric addition process in a tissue-like cell construct under mechanical overload via TPEF-SHG imaging system

Ailin Wei, Zhonghai Wang, Zongming Yang, Shenghao Tan, Tong Ye, Yonghong Shao, Thomas Borg, Bruce Gao

Proceedings Volume 11244, 112442E (2020) https://doi.org/10.1117/12.2546280

KEYWORDS: Two photon excitation microscopy, Imaging systems, Heart, Beam splitters, Second-harmonic generation, Tissues, Microscopes

Read Abstract +

Proceedings Article | 19 April 2017 Presentation

Polarization-resolved SHG microscopy in cardiac hypertrophy study (Conference Presentation)

Zhonghai Wang, Cai Yuan, Yonghong Shao, Amy Bradshaw, Thomas Borg, Bruce Gao

Proceedings Volume 10042, 100420M (2017) https://doi.org/10.1117/12.2253161

KEYWORDS: Medical research, Heart, Tissues, Microscopy, Second-harmonic generation, Polarization, Harmonic generation, Visualization, Molecular biology, Diagnostics

Read Abstract +

Cardiac hypertrophy, a process initiated by mechanical alterations, is hypothesized to cause long-term molecular-level alteration in the sarcomere lattice, which is the main force-generating component in the heart muscle. This molecular-level alteration is beyond the resolving capacity of common light microscopy. Second harmonic generation (SHG) microscopy has unique capability for visualizing ordered molecular structures in biological tissues without labeling. Combined with polarization imaging technique, SHG microscopy is able to extract structural details of myosin at the molecular level so as to reveal molecular-level alterations that occur during hypertrophy. The myosin filaments are believed to possess C6 symmetry; thus, the nonlinear polarization response relationship between generated second harmonic light I^2ωand incident fundamental light I^ω is determined by nonlinear coefficients, χ_15, χ_31 and χ_33. χ_31/χ_15 is believed to be an indicator of the molecular symmetry of myosin filament, whileχ_33/χ_15represents the intramyosin orientation angle of the double helix. By changing the polarization of the incident light and evaluating the corresponding SHG signals, the molecular structure of the myosin, reflected by the χ coefficients, can be revealed. With this method, we studied the structural properties of heart tissues in different conditions, including those in normal, physiologically hypertrophic (heart tissue from postpartum female rats), and pathologically hypertrophic (heart tissue from transverse-aorta constricted rats) conditions. We found that ratios of χ_31/χ_15 showed no significant difference between heart tissues from different conditions; their values were all close to 1, which demonstrated that Kleinman symmetry held for all conditions. Ratios of χ_33/χ_15 from physiologically or pathologically hypertrophic heart tissues were raised and showed significant difference from those from normal heart tissues, which indicated that the intramyosin orientation angle of the double helix was altered when heart tissues hypertrophied. Polarization-resolved SHG microscopy permitted us to study heart tissues at the molecular level and may serve as a diagnostic tool for cardiac hypertrophy.

Proceedings Article | 28 February 2014 Paper

Pulse splitter-based nonlinear microscopy for live-cardiomyocyte imaging

Zhonghai Wang, Wan Qin, Yonghong Shao, Siyu Ma, Thomas Borg, Bruce Gao

Proceedings Volume 8948, 89482X (2014) https://doi.org/10.1117/12.2041845

KEYWORDS: Second-harmonic generation, Imaging systems, Beam splitters, Microscopy, Microscopes, Mirrors, Objectives, Laser safety, Cell mechanics, Electronic filtering

Read Abstract +

Proceedings Article | 26 February 2013 Paper

Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation

Honghai Liu, Wan Qin, Yonghong Shao, Zhonghai Wang, Huaxiao Yang, Raymond Runyan, Thomas Borg, Bruce Gao

Proceedings Volume 8587, 85871W (2013) https://doi.org/10.1117/12.2005156

KEYWORDS: Imaging systems, Second-harmonic generation, Tissues, Heart, In vitro testing, Multichannel imaging systems, Signal detection, Luminescence, Carbon dioxide, Microscopes

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years