Wavelength-dependent measurement of contrast in NIR and extended NIR spectral range (650-1600 nm) in phantoms

Daniel Salo; David M. Kim; Mikhail Y. Berezin

doi:10.1117/12.2044872

17 March 2014 Wavelength-dependent measurement of contrast in NIR and extended NIR spectral range (650-1600 nm) in phantoms

Daniel Salo, David M. Kim, Mikhail Y. Berezin

Author Affiliations +

Proceedings Volume 8940, Optical Biopsy XII; 89400O (2014) https://doi.org/10.1117/12.2044872
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

In order to identify the optimal imaging conditions for the highest contrast in biological tissue, we explored the optical contrast of a phantom as a function of depth and wavelengths of excitation. Our customized optical hardware featured a scanning microscope, and imaging spectrographs equipped with silicon and InGaAs CCD diode array detectors allowed directed comparison of the intensity of NIR (650-900 nm) and exNIR (1000-1600 nm) light transmitted through a phantom (milk). We demonstrated that the contrast depends on the phantom thickness and the wavelength. At low depths (less than 3 mm) NIR light provides the best contrast while exNIR light shows significantly higher contrast in phantoms thicker than 4.5 mm. Our results suggest that distinguishing biological features in deep tissue may benefit from the application of the exNIR for in vivo.

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Daniel Salo, David M. Kim, and Mikhail Y. Berezin "Wavelength-dependent measurement of contrast in NIR and extended NIR spectral range (650-1600 nm) in phantoms", Proc. SPIE 8940, Optical Biopsy XII, 89400O (17 March 2014); https://doi.org/10.1117/12.2044872

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