Holographic quantitative imaging of sample hidden by turbid medium or occluding objects

V. Bianco; L. Miccio; F. Merola; P. Memmolo; O. Gennari; Melania Paturzo; P. A. Netti; P. Ferraro

doi:10.1117/12.2086808

11 March 2015 Holographic quantitative imaging of sample hidden by turbid medium or occluding objects

V. Bianco, L. Miccio, F. Merola, P. Memmolo, O. Gennari, Melania Paturzo, P. A. Netti, P. Ferraro

Proceedings Volume 9336, Quantitative Phase Imaging; 933612 (2015) https://doi.org/10.1117/12.2086808
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Digital Holography (DH) numerical procedures have been developed to allow imaging through turbid media. A fluid is considered turbid when dispersed particles provoke strong light scattering, thus destroying the image formation by any standard optical system. Here we show that sharp amplitude imaging and phase-contrast mapping of object hidden behind turbid medium and/or occluding objects are possible in harsh noise conditions and with a large field-of view by Multi-Look DH microscopy. In particular, it will be shown that both amplitude imaging and phase-contrast mapping of cells hidden behind a flow of Red Blood Cells can be obtained. This allows, in a noninvasive way, the quantitative evaluation of living processes in Lab on Chip platforms where conventional microscopy techniques fail. The combination of this technique with endoscopic imaging can pave the way for the holographic blood vessel inspection, e.g. to look for settled cholesterol plaques as well as blood clots for a rapid diagnostics of blood diseases.

Citation Download Citation

V. Bianco, L. Miccio, F. Merola, P. Memmolo, O. Gennari, Melania Paturzo, P. A. Netti, and P. Ferraro "Holographic quantitative imaging of sample hidden by turbid medium or occluding objects", Proc. SPIE 9336, Quantitative Phase Imaging, 933612 (11 March 2015); https://doi.org/10.1117/12.2086808

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