Fast photoacoustic imaging with a line scanning optical-acoustical resolution photoacoustic microscope (LS-OAR-PAM)

Robert Nuster; Guenther Paltauf

doi:10.1117/12.2183743

16 July 2015 Fast photoacoustic imaging with a line scanning optical-acoustical resolution photoacoustic microscope (LS-OAR-PAM)

Robert Nuster, Guenther Paltauf

Author Affiliations +

Proceedings Volume 9539, Opto-Acoustic Methods and Applications in Biophotonics II; 95390S (2015) https://doi.org/10.1117/12.2183743
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

We present the concept, the setup and a preliminary experiment using optical ultrasound detection with a CCD camera combined with focused line excitation for photoacoustic microscopy. The line scanning optical-acoustical resolution photoacoustic microscope (LS-OAR-PAM) with optical ultrasound detection is capable of real-time B-scan imaging providing acoustical resolution within the individual B-scans and optical out of plane resolution up to a depth limited by optical diffusion. A 3D image is composed of reconstructed B-scan images recorded while scanning the excitation line along the sample surface. Proof of concept is shown by imaging a phantom containing black human hairs and carbon fibers. The obtained C-scan image clearly shows the different resolution in the two perpendicular directions, namely diffraction limited by optical focusing in scan direction and acoustically limited in direction parallel to line orientation by the properties of acoustic wave propagation.

Citation Download Citation

Robert Nuster and Guenther Paltauf "Fast photoacoustic imaging with a line scanning optical-acoustical resolution photoacoustic microscope (LS-OAR-PAM)", Proc. SPIE 9539, Opto-Acoustic Methods and Applications in Biophotonics II, 95390S (16 July 2015); https://doi.org/10.1117/12.2183743

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