Nonlinear imaging histopathology: a method to correlate the gold-standard hematoxylin and eosin staining with modern nonlinear microcopy

Kayvan F. Tehrani; Jaena Park; Eric J. Chaney; Haohua Tu; Stephen A. Boppart

doi:10.1117/12.2648062

6 March 2023 Nonlinear imaging histopathology: a method to correlate the gold-standard hematoxylin and eosin staining with modern nonlinear microcopy

Kayvan F. Tehrani, Jaena Park, Eric J. Chaney, Haohua Tu, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12368, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI; PC123680C (2023) https://doi.org/10.1117/12.2648062
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Hematoxylin and eosin (H&E) staining in histology, the century-old technique, has been the gold standard tool for pathologists to detect anomalies in tissues and detect disorders such as cancer. Even in the modern era, several new label-free imaging techniques have been developed which provide many more precise layers of information, but they have yet to translate to the clinic, mainly due to a lack of direct comparison between these techniques. Here we present the use of laser ablation to produce fiduciary markers to enable the correlation of nonlinear microscopy with the gold-standard tool of histology.

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Citation Download Citation

Kayvan F. Tehrani, Jaena Park, Eric J. Chaney, Haohua Tu, and Stephen A. Boppart "Nonlinear imaging histopathology: a method to correlate the gold-standard hematoxylin and eosin staining with modern nonlinear microcopy ", Proc. SPIE PC12368, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI, PC123680C (6 March 2023); https://doi.org/10.1117/12.2648062

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