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25 September 2013 Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin
Te-Yu Tseng, Hsun-Chia Hsu, Chiu-Sheng Yang, Tsung-Hua Tsai, Chen-Yuan Dong
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Journal of Biomedical Optics, Vol. 18, Issue 9, 096016 (September 2013). https://doi.org/10.1117/1.JBO.18.9.096016
Abstract
Transdermal drug delivery, transporting the drug molecules through epidermis to dermis, has been extensively investigated but not studied in dynamic detail. The objective of this study is to monitor the dynamical changes of drug permeation and that of polarization of skin tissue with oleic acid treatment. We utilize two-photon fluorescence microscopy (TPM) to investigate the dynamics of transdermal drug delivery in skin excised from the abdominal region of euthenized nude mice with sulforhodamine B (SRB) modeling as a drug and Laurdan serving as a polarity indicator. The treatment of oleic acid increases the permeation rate of SRB, quickly reaching to the steady state of permeation. Increases in polarity within the skin tissue (in both intercellular and intracellular region of stratum corneum) are observed in SRB permeation enhanced by oleic acid treatment. TPM has successfully demonstrated the ability to study spatial distribution of transport dynamics in oleic acid-enhanced transdermal delivery.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Te-Yu Tseng, Hsun-Chia Hsu, Chiu-Sheng Yang, Tsung-Hua Tsai, and Chen-Yuan Dong "Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin," Journal of Biomedical Optics 18(9), 096016 (25 September 2013). https://doi.org/10.1117/1.JBO.18.9.096016
Published: 25 September 2013
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Cited by 2 scholarly publications.
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KEYWORDS
Skin
Molecules
Luminescence
Tissues
Two photon excitation microscopy
3D image processing
Photons
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