Optimization of sample cooling temperature for redox cryo-imaging

Anle Wang; Jing Yuan; Weihua Luo; Mengmeng Liu; Qingming Luo

doi:10.1117/1.JBO.19.8.080502

22 August 2014 Optimization of sample cooling temperature for redox cryo-imaging

Anle Wang, Jing Yuan, Weihua Luo, Mengmeng Liu, Qingming Luo

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Journal of Biomedical Optics, Vol. 19, Issue 8, 080502 (August 2014). https://doi.org/10.1117/1.JBO.19.8.080502

Abstract

Cryo-imaging techniques have been widely used to measure the metabolic state of tissues by capturing reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) autofluorescence. However, NADH and FAD fluorescence is sensitive to changes in temperature, which may result in unreliable redox ratio calculations. Here, the relationship between the measured redox ratio and sample surface temperature was analyzed using a standard phantom solution and biological tissues. The results indicated that a temperature <−100°C was a suitable cryo-imaging temperature window in which redox ratio measuring was immune to temperature fluctuations. These results may serve as a reference for designing and optimizing redox cryo-imaging experiments for quantitatively mapping the metabolic state of biological samples.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Anle Wang, Jing Yuan, Weihua Luo, Mengmeng Liu, and Qingming Luo "Optimization of sample cooling temperature for redox cryo-imaging," Journal of Biomedical Optics 19(8), 080502 (22 August 2014). https://doi.org/10.1117/1.JBO.19.8.080502

Published: 22 August 2014

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