Label-free nanoscale characterization of red blood cell structure and dynamics using single-shot transport of intensity equation

Praveen Kumar Poola; Renu John

doi:10.1117/1.JBO.22.10.106001

5 October 2017 Label-free nanoscale characterization of red blood cell structure and dynamics using single-shot transport of intensity equation

Praveen Kumar Poola, Renu John

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Journal of Biomedical Optics, Vol. 22, Issue 10, 106001 (October 2017). https://doi.org/10.1117/1.JBO.22.10.106001

Abstract

We report the results of characterization of red blood cell (RBC) structure and its dynamics with nanometric sensitivity using transport of intensity equation microscopy (TIEM). Conventional transport of intensity technique requires three intensity images and hence is not suitable for studying real-time dynamics of live biological samples. However, assuming the sample to be homogeneous, phase retrieval using transport of intensity equation has been demonstrated with single defocused measurement with x-rays. We adopt this technique for quantitative phase light microscopy of homogenous cells like RBCs. The main merits of this technique are its simplicity, cost-effectiveness, and ease of implementation on a conventional microscope. The phase information can be easily merged with regular bright-field and fluorescence images to provide multidimensional (three-dimensional spatial and temporal) information without any extra complexity in the setup. The phase measurement from the TIEM has been characterized using polymeric microbeads and the noise stability of the system has been analyzed. We explore the structure and real-time dynamics of RBCs and the subdomain membrane fluctuations using this technique.

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Praveen Kumar Poola and Renu John "Label-free nanoscale characterization of red blood cell structure and dynamics using single-shot transport of intensity equation," Journal of Biomedical Optics 22(10), 106001 (5 October 2017). https://doi.org/10.1117/1.JBO.22.10.106001

Received: 18 April 2017; Accepted: 23 August 2017; Published: 5 October 2017

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