Redox ratio of mitochondria as an indicator for the response of photodynamic therapy

Zhihong Zhang; Dana Blessington; Hui Li; Theresa M. Busch; Jerry D. Glickson; Qingming Luo; Britton Chance; Gang Zheng

doi:10.1117/1.1760759

1 July 2004 Redox ratio of mitochondria as an indicator for the response of photodynamic therapy

Zhihong Zhang, Dana Blessington, Hui Li, Theresa M. Busch, Jerry D. Glickson, Qingming Luo, Britton Chance, Gang Zheng

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Journal of Biomedical Optics, Vol. 9, Issue 4, (July 2004). https://doi.org/10.1117/1.1760759

Abstract

The effect of photodynamic therapy (PDT) treatment on the metabolic state of tumor mitochondria is investigated by imaging of tumor redox status. PDT is performed using the photosensitizer pyropheophorbide-2-deoxyglucosamide (Pyro-2DG), which utilizes the glucose import pathway. It is found that Pyro-2DG-induced PDT resulting in a highly oxidized state of tumor mitochondria. This is determined from the redox ratio changes derived from the intrinsic oxidized flavoprotein (Fp) and reduced pyridine nucleotide (PN) [i.e., reduced nicotinamide adenine dinucleotide (NADH)] fluorescence signals observed using a cryoimager. Thus, the redox ratio is a sensitive indicator for providing reliable and informative measurements of PDT-induced tissue damage. In the PDT treated region of the tumor, highly oxidized flavoprotein and diminishing NADH fluorescence is detected, suggesting that flavoprotein and NADH are oxidized by singlet oxygen produced in the photosensitization process.

©(2004) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Zhihong Zhang, Dana Blessington, Hui Li, Theresa M. Busch, Jerry D. Glickson, Qingming Luo, Britton Chance, and Gang Zheng "Redox ratio of mitochondria as an indicator for the response of photodynamic therapy," Journal of Biomedical Optics 9(4), (1 July 2004). https://doi.org/10.1117/1.1760759

Published: 1 July 2004

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