Ms. Ashwini S. Galande Profile

Ashwini S. Galande

at Indian Institute of Technology Hyderabad

SPIE Involvement:

Author

Publications (3)

SPIE Journal Paper | 30 January 2024 Open Access

TIE-GANs: single-shot quantitative phase imaging using transport of intensity equation with integration of GANs

Vikas Thapa, Ashwini Galande, Gurram Hanu Phani Ram, Renu John

JBO, Vol. 29, Issue 01, 016010, (January 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.1.016010

KEYWORDS: Education and training, Gallium nitride, Phase reconstruction, Phase imaging, Image restoration, Microscopes, Data modeling, Biomedical optics, Phase retrieval, Biological samples

Read Abstract +

SignificanceArtificial intelligence (AI) has become a prominent technology in computational imaging over the past decade. The expeditious and label-free characteristics of quantitative phase imaging (QPI) render it a promising contender for AI investigation. Though interferometric methodologies exhibit potential efficacy, their implementation involves complex experimental platforms and computationally intensive reconstruction procedures. Hence, non-interferometric methods, such as transport of intensity equation (TIE), are preferred over interferometric methods.AimTIE method, despite its effectiveness, is tedious as it requires the acquisition of many images at varying defocus planes. The proposed methodology holds the ability to generate a phase image utilizing a single intensity image using generative adversarial networks (GANs). We present a method called TIE-GANs to overcome the multi-shot scheme of conventional TIE.ApproachThe present investigation employs the TIE as a QPI methodology, which necessitates reduced experimental and computational efforts. TIE is being used for the dataset preparation as well. The proposed method captures images from different defocus planes for training. Our approach uses an image-to-image translation technique to produce phase maps and is based on GANs. The main contribution of this work is the introduction of GANs with TIE (TIE:GANs) that can give better phase reconstruction results with shorter computation times. This is the first time the GANs is proposed for TIE phase retrieval.ResultsThe characterization of the system was carried out with microbeads of 4 μm size and structural similarity index (SSIM) for microbeads was found to be 0.98. We demonstrated the application of the proposed method with oral cells, which yielded a maximum SSIM value of 0.95. The key characteristics include mean squared error and peak-signal-to-noise ratio values of 140 and 26.42 dB for oral cells and 100 and 28.10 dB for microbeads.ConclusionsThe proposed methodology holds the ability to generate a phase image utilizing a single intensity image. Our method is feasible for digital cytology because of its reported high value of SSIM. Our approach can handle defocused images in such a way that it can take intensity image from any defocus plane within the provided range and able to generate phase map.

DOWNLOAD PAPER

Proceedings Article | 9 August 2023 Paper

Low-cost portable lens less digital holographic microscope for studying anemic RBCs

Aswathy Vijay, Ashwini Subhash Galande, Renu John

Proceedings Volume 12630, 1263016 (2023) https://doi.org/10.1117/12.2670549

KEYWORDS: 3D image reconstruction, Red blood cells, Holography, Digital holography, Holograms, Biological samples, Image restoration, Reconstruction algorithms, Phase reconstruction, Microscopes

Read Abstract +

SPIE Journal Paper | 21 October 2020

Nanometric depth phase imaging using low-cost on-chip lensless inline holographic microscopy

Hanu Phani Ram Gurram, Ashwini Galande, Renu John

OE, Vol. 59, Issue 10, 104105, (October 2020) https://doi.org/10.1117/12.10.1117/1.OE.59.10.104105

KEYWORDS: Holograms, 3D image reconstruction, Microscopes, Reconstruction algorithms, Holography, Signal to noise ratio, Phase imaging, Microscopy, Phase retrieval, Optical engineering

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years