The purpose of this study was to investigate which illumination arrangement can provide the highest image quality when
using a non-invasive cutaneous imaging system to observe capillaries in nail-fold. We captured the microcirculation
images with and without a band-pass filter which wavelength is 556 ± 10 nm in front of a 150 Watt halogen lamp.
Furthermore, we varied the illumination angle form 90 degree (co-axial light) to 20 degree to evaluate the image quality
under different light source arrangements. The image registration function was used to solve the image movement
problem which is due to breathing or slightly movement of volunteers or the imaging device. The contrast-to-noise ratio
(CNR) is an evaluation factor to quantify the image quality. The dynamic search method was used to find out the
skeleton of a vessel and define the foreground and background parameters for calculating contrast. A Gaussian smooth
filter was applied to the original images and noise was resulted from differentiating the coefficient of variance (CV) of
the original and processed images. As a result, using a green filter in front of a lamp presents the highest
contrast-to-noise ratio when the illumination angle is 20 degree. By normalizing the highest CNR to 1, the CNR of other
illumination conditions are 0.49 (without filter, 20 degree), 0.39 (with filter, 90 degree) and 0.28 (without filter, 90
degree), respectively. It is concluded that using a green light source with an illuminating angle of 20 degree will provide
better image quality than other arrangements.
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