The design of a plastic optical fiber-based surface plasmon resonance biochip has been developed and their theoretical and experimental aspects are presented here. Multilayer Fresnel equations combined with an available experimental database are used to verify the SPR effect over different metal thin film and substrate materials, considering the sensor immersed in an aqueous solution. Also, the optical substrate preparation with the cladding removing and experimental aspects are presented and discussed. Only angular interrogation mode has been observed and finally an experimental setup has been proposed.
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