We investigated the “laser adjuvant” that improved vaccine efficacy, via combining non-invasive near-infrared red (NIR) light irradiation and intradermal influenza vaccine and discovered the crucial role of reactive oxygen species (ROS) and mast cells. This study elucidates the effects of a new wavelength (1270 nm) of NIR light that exhibits a similar adjuvant effect at lower energy on mast cells and mouse skin, revealing ROS and ATP production via mitochondrial photoreception, increased chemokine mRNA expression, and durable antibody titers in immunized mice. These findings highlight the clinical potential of 1270 nm NIR light, demonstrating the wavelength-specific effect of laser adjuvants.
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