Bacterial antibiotic resistance poses a pressing global health crisis, challenging conventional therapies. Efflux pumps diminish antimicrobial effectiveness by expelling drugs from bacterial cells. Multidrug efflux pumps (MEPs) have been found to transport diverse compounds, including phenothiazinium dyes like methylene blue, out of bacteria. Inhibition of MEPs offers a promising strategy to bolster the efficacy of antimicrobial photodynamic inactivation (PDT). This research adopts a synergistic approach, combining the efflux pump inhibitor (EPI) , reserpine, with silver nanoparticles (Ag NPs) and methylene blue (MB) to enhance PDT efficiency. Ag NPs were synthesized via pulsed laser ablation and characterized using TEM, UV-Vis, and PL spectra. E. coli was treated with MB, Ag NPs, and reserpine, followed by LED light irradiation. MB was twice as effective, and AgNPs/MB was six times more effective with reserpine during a sixminute irradiation. Ongoing experiments on morphological changes will be presented. AgNPs/MB with reserpine could effectively combat bacterial pathogens in open wounds and prosthetic joint infections.
This conference presentation was prepared for the Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXI conference at SPIE BiOS, SPIE Photonics West 2023.
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