It is difficult to introduce a specific amount of a substance into cells by existing injection methods because there is no
appropriate method of directly measuring the quantity of the injected substance. Although radioisotopes can be used,
there is currently no apparatus that can practically handle such radioisotopes. The measurement of the diameter of a
liquid droplet in air or oil is affected by surface tension if the liquid droplet is very small; but this issue does not occur
with microinjection, in which a water solution is discharged under pressure through a capillary and into a cell. It is also
difficult to measure the density or mass of the injected substance because of the low discharge rate, unlike the case of
inkjet printers. To solve these problems, we propose a method of precise microinjection by summation of fluorescence
intensity. In addition, we developed a new pressure pulse injection device that generates pressure with a rectangular
waveform and a precise amplitude and pulse width to improve controllability of the discharge amount. Lastly, when the
above device and method are combined, the coefficient of correlation between the specified number of pressure pulses
per unit of time and the actual discharge amount exceeded 0.999. This research paper describes in detail the measurement
system, standalone performance, and quantities of substances introduced into living cells.
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