Mr. Marcus Kwon Profile

Marcus Kwon

at Oregon Health & Science Univ

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Publications (2)

SPIE Journal Paper | 18 April 2023 Open Access

Dual probe difference specimen imaging for prostate cancer margin assessment

Marcus J. Kwon, Broderick J. House, Connor W. Barth, Allison Solanki, Jocelyn A. Jones, Scott C. Davis, Summer L. Gibbs

JBO, Vol. 28, Issue 08, 082806, (April 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.8.082806

KEYWORDS: Tumors, Adipose tissue, Prostate cancer, Prostate, Muscles, Tissues, Resection, Antibodies, Fluorescence, Cancer

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SignificancePositive margin status due to incomplete removal of tumor tissue during radical prostatectomy for high-risk localized prostate cancer requires reoperation or adjuvant therapy, which increases morbidity and mortality. Adverse effects of prostate cancer treatments commonly include erectile dysfunction, urinary incontinence, and bowel dysfunction, making successful initial curative prostatectomy imperative.AimCurrent intraoperative tumor margin assessment is largely limited to frozen section analysis, which is a lengthy, labor-intensive process that is obtrusive to the clinical workflow within the operating room (OR). Therefore, a rapid method for prostate cancer margin assessment in the OR could improve outcomes for patients.ApproachDual probe difference specimen imaging (DDSI), which uses paired antibody-based probes that are labeled with spectrally distinct fluorophores, was shown herein for prostate cancer margin assessment. The paired antibody-based probes consisted of a targeted probe to prostate-specific membrane antigen (PSMA) and an untargeted probe, which were used as a cocktail to stain resected murine tissue specimens including prostate tumor, adipose, muscle, and normal prostate. Ratiometric images (i.e., DDSI) of the difference between targeted and untargeted probe uptake were calculated and evaluated for accuracy using receiver operator characteristic curve analysis with area under the curve values used to evaluate the utility of the DDSI method to detect PSMA positive prostate cancer.ResultsTargeted and untargeted probe uptake was similar between the high and low PSMA expressing tumor due to nonspecific probe uptake after topical administration. The ratiometric DDSI approach showed substantial contrast difference between the PSMA positive tumors and their respective normal tissues (prostate, adipose, muscle). Furthermore, DDSI showed substantial contrast difference between the high PSMA expressing tumors and the minimally PSMA expressing tumors due to the ratiometric correction for the nonspecific uptake patterns in resected tissues.ConclusionsPrevious work has shown that ratiometic imaging has strong predictive value for breast cancer margin status using topical administration. Translation of the ratiometric DDSI methodology herein from breast to prostate cancers demonstrates it as a robust, ratiometric technique that provides a molecularly specific imaging modality for intraoperative margin detection. Using the validated DDSI protocol on resected prostate cancers permitted rapid and accurate assessment of PSMA status as a surrogate for prostate cancer margin status. Future studies will further evaluate the utility of this technology to quantitatively characterize prostate margin status using PSMA as a biomarker.

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Proceedings Article | 7 March 2021 Presentation

Water-soluble and cell-permeant zwitterionic rhodamine dyes enable quantitative imaging of drug target engagement

Antonio Montaño, Lei Wang, Allison Solanki, Nathan McMahon, Marcus Kwon, Kenneth Tichauer, Kimberley Samkoe, Summer Gibbs

Proceedings Volume 11624, 116240H (2021) https://doi.org/10.1117/12.2582638

KEYWORDS: Rhodamine, Cancer, Resistance, Molecules, Imaging systems, Visualization, Silicon, Receptors, Oncology, Dynamical systems

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