The article presents the results of a study of the possibilities of 3D Stokes-polarimetric mapping of microscopic images of protein fluorofors of the prostate. Polarization-holographic measurement and analysis of layer-by-layer maps and histograms of the distribution of the polarization ellipticity of microscopic images of biological preparations of the prostate. Determination of the relationship between statistical moments of the 1st - 4th orders, which characterize layer-by-layer maps of distributions of the polarization ellipticity of microscopic images of biological preparations of the prostate and pathological conditions of the prostate. Determination of the operational characteristics (sensitivity, specificity, accuracy) of the diagnostic strength of the 3D layer-by-layer Stokes polarimetric mapping method.
The results of algorithmic testing of digital histological studies of the duration of kidney tissue damage based on polarization reconstruction of linear birefringence maps of protein fibrillar networks are presented. The relationships between the temporary change in the magnitude of the statistical moments of the 1st - 4th orders of magnitude characterizing the distribution of the degree of crystallization of histological sections of the kidney and the duration of damage are determined.
KEYWORDS: Data modeling, Statistical modeling, Polarization, Microscopy, Machine learning, Data processing, Tissues, Information technology, Intelligence systems
The work combines methods of multidimensional polarization microscopy, statistical processing of data and algorithms of machine learning with the purpose of constructing a methodology for creation of intelligent systems for multi-level medical monitoring of joint lesions . The task of classifying the results of the study of biological materials for obtaining a diagnosis was solved. To obtain informative features, a model of biological tissue was developed and the main diagnostic parameters were determined (statistical moments of 1-4 orders of coordinate distributions of the values of azimuths and the ellipticity of polarization and their autocorrelation functions, as well as wavelet coefficients of the corresponding distributions). The classification of these data was provided on the raw input data and on generated data with different degree of overlapping classes by machine learning algorithms and inductive modeling.
A theoretical basis for the method of polarization-interference mapping of optically thin polycrystalline films of human biological fluids is given. The coordinate distributions of the value of the local contrast of the interference distributions of the polarization-inhomogeneous microscopic images of polycrystalline films of the synovial fluid of the human joint are investigated. In the framework of the statistical (statistical moments of the 1st-4th order) approaches, objective criteria for the distribution of the values of local contrast are established. The possibility of differentiation of weak changes in the optical anisotropy of blood films of healthy and patients with breast cancer patients is determined.
This paper contains structural and logical scheme of the research; theoretical information about the set of azimuthally invariant Mueller-matrix elements and their combinations; The work is aimed at the development of a set of techniques that form a new method of azimuthally invariant differential polarimetry of partially-depolarizing optically anisotropic biological layers. This method is based on the determination and diagnostic use of a set of physical relationships between the distributions of azimuthally invariant polarization parameters characterizing the optical anisotropy of partially depolarizing layers of biological tissues, and the distributions of the parameters of linear and circular birefringence of such objects.
Experimental studies within the statistical approach of the coordinate structure of the distributions of the intensity of own fluorescence of polycrystalline blood plasma films of patients of the following groups: control group of donors - group 1; patients with non-alcoholic fatty liver disease - group 2; patients with chronic hepatitis - group 3: The average values and ranges of variation of statistical moments of the 1st - 4th orders determined within the representative samples, which characterize the coordinate distributions of the intensity values of autofluorescent microscopic images of samples of polycrystalline blood plasma films within groups 1, 2, 3. The analysis of the operating characteristics of the power of the method of laser polarization mapping of two-dimensional distributions of the intensity values of its own fluorescence of microscopic images based on the determination of the sensitivity values, specificity and accuracy of the diagnostic test.
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