To promote the application of sampling moiré method, in this study, the determination of the moiré spacing in sampling moiré method is studied in details. The ordinary equation for determining the sampling moiré spacing is found to be inapplicable in some cases, then a new equation is derived based on the generation mechanism of sampling moiré. It is found that the ordinary equation is a particular form of the new equation in a specific range. Besides, simulation tests demonstrate that the new equation has a wide application range.
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