Stimulated Raman scattering (SRS) microscopy is a powerful technique that allows for the visualization of molecular vibrational images for label-free imaging, metabolic imaging, and supermultiplex imaging. However, its sensitivity is mainly limited by the shot noise of laser pulses, and long acquisition times are sometimes required to detect weak SRS signals hidden by the shot noise. To overcome this limitation, quantum-enhanced (QE-) SRS microscopy has been demonstrated, while the low optical power of squeezed pulses limits the sensitivity. In this work, we present QE-SRS microscopy using quantum-enhanced balanced detection (QE-BD) scheme, where the sensitivity of balanced detection SRS microscope is enhanced by injecting squeezed vacuum, allowing for QE-SRS imaging with high power SRS pump pulses (typically several tens of milliwatt), while balanced detection causes 3 dB drawback in the signal-to-noise ratio. We experimentally demonstrate QE-SRS imaging with 2.6 dB noise reduction compared with shot-noise-limited balanced detection SRS. We also demonstrate hyperspectral QE-SRS imaging by fast wavelength tuning of Stokes pulses. These results show the potential feasibility of high-power QE-SRS whose sensitivity is beyond that of classical shot-noise-limited SRS microscopes.
The stratum corneum (SC), the outermost layer of the skin, plays a vital role in providing a barrier against dry environments. In order to understand the barrier function and overall condition of the skin, it is essential to assess the ability of the SC to absorb and retain water. In this study, we used stimulated Raman scattering (SRS) imaging to investigate the threedimensional SC structure and water distribution as water was absorbed into dried SC sheets. Our findings indicate that the process of water absorption and retention is dependent on the specific sample and can be spatially heterogeneous. These results demonstrate the potential of SRS imaging in diagnosing skin conditions.
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