We present an ambient light-compatible wide-field fluorescence-guided surgery imaging platform for real-time, near-infrared imaging to assess vascular perfusion and flap viability in free flap breast reconstruction surgery. The platform allowed simultaneous white-light and fluorescence imaging, and was used to capture high-resolution and high dynamic range intraoperative images and videos for rapid assessment of tissue perfusion. All data were captured with room lights on with minimal interruption to the surgical workflow. Postoperative image analysis demonstrates the ability of the OnLume wide-field Fluorescence-Guided Surgery (FGS) Imaging System to provide robust imagery that enhances surgical assessment of the viability of the autologous flap for breast reconstruction.
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