Mr. Vikram Kohli Profile

Vikram Kohli

at Univ of Alberta

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Author

Publications (5)

Proceedings Article | 20 February 2008 Paper

Part I. Embryonic surgery using femtosecond laser pulses for the delivery of exogenous materials and the analysis of gene expression

V. Kohli, A. Elezzabi

Proceedings Volume 6854, 685413 (2008) https://doi.org/10.1117/12.760078

KEYWORDS: Femtosecond phenomena, Quantum dots, Luminescence, Interfaces, Laser development, Laser therapeutics, Cavitation, Surgery, Molecules, Green fluorescent protein

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Proceedings Article | 20 February 2008 Paper

Part II: Morphological analysis of embryonic development following femtosecond laser manipulation

V. Kohli, A. Elezzabi

Proceedings Volume 6854, 685404 (2008) https://doi.org/10.1117/12.760082

KEYWORDS: Luminescence, Scanning electron microscopy, Femtosecond phenomena, Laser development, Pulsed laser operation, Beam controllers, Ear, Laser ablation, Cavitation, Morphological analysis

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Proceedings Article | 14 February 2008 Paper

From cells to embryos: the application of femtosecond laser pulses for altering cellular material in complex biological systems

V. Kohli, A. Elezzabi

Proceedings Volume 6892, 68920J (2008) https://doi.org/10.1117/12.759687

KEYWORDS: Femtosecond phenomena, Luminescence, Quantum dots, Laser development, Scanning electron microscopy, Interfaces, Laser therapeutics, Surgery, Microscopes, Plasma

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We report the application of high-intensity femtosecond laser pulses as a novel tool for manipulating biological specimens. When femtosecond laser pulses were focused to a near diffraction-limited focal spot, cellular material within the laser focal volume was surgically ablated. Several dissection cuts were made in the membrane of live mammalian cells, and membrane surgery was accomplished without inducing cell collapse or disassociation. By altering how the laser pulses were applied, focal adhesions joining live epithelial cells were surgically removed, resulting in single cell isolation. To further examine the versatility of this reported tool, cells were transiently permeabilized for introducing foreign material into the cytoplasm of live mammalian cells. Localizing focused femtosecond laser pulses on the biological membrane resulted in the formation of transient pores, which were harnessed as a pathway for the delivery of exogenous material. Individual mammalian cells were permeabilized in the presence of a hyperosmotic cryoprotective disaccharide. Material delivery was confirmed by measuring the volumetric response of cells permeabilized in 0.2, 0.3, 0.4 and 0.5 M cryoprotective sugar. The survival of permeabilized cells in increasing osmolarity of sugar was assessed using a membrane integrity assay. Further demonstrating the novelty of this reported tool, laser surgery of an aquatic embryo, the zebrafish (Danio rerio), was also performed. Utilizing the transient pores that were formed in the embryonic cells of the zebrafish embryo, an exogenous fluorescent probe FITC, Streptavidin-conjugated quantum dots or plasmid DNA (sCMV) encoding EGFP was introduced into the developing embryonic cells. To determine if the laser induced any short- or long-term effects on development, laser-manipulated embryos were reared to 2 and 7 days post-fertilization and compared to control embryos at the same developmental stages. Light microscopy and scanning electron microscopy were used to compare whole body mosaics of the developed larvae. Key developmental features that were compared included the olfactory pit, dorsal, ventral and pectoral fins, notochord, otic capsule and otic vesicle. No differences in the morphology and placement of the fore-, mid- and hindbrains were observed.

Proceedings Article | 13 March 2007 Paper

Laser-assisted ablation of corneal-like material using focused femtosecond laser pulses

Vikram Kohli, Deli Yu, Abulhakem Elezzabi

Proceedings Volume 6460, 64600D (2007) https://doi.org/10.1117/12.699247

KEYWORDS: Laser ablation, Femtosecond phenomena, Pulsed laser operation, Camera shutters, Laser tissue interaction, Laser energy, Surgery, Tissues, Optical amplifiers, Laser therapeutics

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Proceedings Article | 1 March 2006 Paper

Permeabilization and cell surgery using femtosecond laser pulses: an emerging tool for cellular manipulation

Vikram Kohli, Jason Acker, Abdulhakem Elezzabi

Proceedings Volume 6084, 608414 (2006) https://doi.org/10.1117/12.655618

KEYWORDS: Femtosecond phenomena, Surgery, Diffusion, Laser therapeutics, Molecules, Pulsed laser operation, Molecular lasers, Plasma, Charge-coupled devices, Laser energy

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