Microfluidic technology has made significant strides in biomedical, chemical analysis, and materials science, becoming an indispensable tool in these fields. In this study, we present a novel microfluidic chip featuring a distinctive wavy-like floating electrode structure. During the experimental process, the application of a set of 180° out-of-phase sinusoidal signals to the exciting electrode successfully achieved cell focusing and out-of-plane rotation. By adjusting the parameters of the input exciting signal, precise control over cell focusing and rotation behavior was attained. This design is characterized by its simplicity, operational convenience, and the absence of complex external equipment, offering promising avenues for advancing cellular research.
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