Low frequency vibrations (< 200cm^(-1)) contain the structural information of molecules or the crystal lattice - making low frequency Raman imaging an ideal candidate to analyze heavy molecules, crystal formation etc. The longer integration times of spontaneous Raman spectroscopy, however, limits the study of dynamic structural changes.
In our work, we have built a stimulated Raman scattering (SRS) pump probe scheme combining impulsive excitation with a fast acousto-optics delay line. With a pixel dwell time of 25μs, we have acquired, on a shot noise limited detection system, sub second low frequency (< 200cm^(-1)) hyper-spectral SRS images of various samples.
We present advances in coherent Raman imaging for gastrointestinal cancer detection. We use stimulated Raman scattering (SRS) combined with second harmonic generation (SHG) to reveal cell nuclei, cytoplasm and collagen simultaneously in human tissues. Cell nuclei, cytoplasm and extra cellular matrix can be visualized in real time with image quality similar to conventional histology.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.