In this paper, a holographic lensless quantitative phase imaging (QPI) microscope is presented, which is composed of a CMOS detector image sensor with a programmable color LED matrix, without any lens and mechanical displacement device. Such a miniaturized system can provide a field-portable cost-effective platform for highthroughput quantification of multiple samples. Coordinating the self-developed software operating system, the bright-field imaging, the quantitative phase imaging as well as cell counting, profile analysis, three-dimensional (3D) imaging and differential interference contrast (DIC) imaging can be realized. With its high-resolution based computational microscopy interface, this system can be also adaptively used for telemedicine applications and point-of-care testing (POCT) in resource-limited environments.
Lensless imaging technique, as a newly developed microscopic imaging method, combined with its corresponding image restoration algorithm, and it can obtain large-field, high-resolution three-dimensional images without labeling. Therefore, the lensless imaging system has the advantages of low cost, good portability, large field of view and high resolution. However, due to the limitation of pixel size, hardware implementation and post data processing, the imaging resolution of lensless microscopy is far away from theoretical performance. In order to solve above problems, this paper proposes a method to improve the capability registration and information coupling based on multi-wavelength illumination. Moreover, this work combines a stack of captured low-resolution images into a high-resolution result image, and the pixel super-resolution can be realized throught the reconstruction algorithm based on the exist illumination light source, Finally, experimental results utilizing USAF target demonstrate the success of proposed lensless imaging method.
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