KEYWORDS: In vivo imaging, Nanowires, Optical coherence tomography, Luminescence, Optical tracking, Microscopy, Laser optics, Frequency modulation, Fermium, Regenerative medicine
We demonstrate that the lasing emission spectra of nanowire lasers internalized by progenitor retinal pigmented epithelial cells (RPE) can be exploited as unique “identifiers” to label each individual cell during long-time in vivo observation. Since nanowires could provide a 25 dB signal enhancement in optical coherence tomography (OCT) and green emission in fluorescence microscopy (FM), we utilized OCT and FM concurrently to track the 3D trajectories of RPE cells in rabbit retina in vivo migrating towards the laser-induced wounds. Our study confirms the feasibility of nanowire lasers as novel probes in single progenitor cell tracking, which could potentially facilitate the fundamental research in regenerative medicine.
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