Ms. Xin Qi Profile

Xin Qi

at Shandong Univ

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 1 November 2021 Paper

Detection of microparticles in flow by 2D light scattering and fluorescence imaging

Xin Qi, Zhuo Wang, Xuantao Su

Proceedings Volume 12057, 120573J (2021) https://doi.org/10.1117/12.2606539

KEYWORDS: Light scattering, Particles, Mie scattering, Microfluidics, Sensors, Medical research

Read Abstract +

The in-flow detection of microparticles is of great importance in biomedical fields. Previous studies have shown that twodimensional (2D) light scattering technology has the capability to detect and differentiate unlabeled cells, while fluorescence imaging can be used as a specific cytological detection method. Here, we built a system to acquire simultaneously the 2D light scattering patterns and fluorescence images of single microparticles in flow. A 488 nm laser beam was reshaped into a light sheet by a cylindrical lens, which was used to excite the microparticles in a microfluidic tube. The 2D light scattering and fluorescence signals were collected by a 10x objective lens through a 505 nm dichroic beam splitter, and they were simultaneously acquired by dual complementary metal oxide semiconductor (CMOS) detectors. Our system acquired the 2D light scattering patterns and fluorescence images of single microparticles from a mixture (2 μm fluorescent particles, 2 μm and 3.87 μm non-fluorescent particles) in flow. Based on the 2D light scattering patterns and the fluorescence images, the number and the ratio of mixed microparticles can be determined. Moreover, the label-free microparticle size differentiation can also be achieved with the 2D light scattering patterns. The detection of microparticles in flow that combines label-free 2D light scattering technology and fluorescence imaging may find potential applications for single cell analysis.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years