Combined IR and 31P NMR method for determining fractional composition in binary phospholipid monolayer films. Applications to phosphocholine: Phosphoglycerol mixtures

Amy D. Williams; Richard A. Dluhy

doi:10.1117/12.166697

31 January 1994 Combined IR and ³¹P NMR method for determining fractional composition in binary phospholipid monolayer films. Applications to phosphocholine: Phosphoglycerol mixtures

Amy D. Williams, Richard A. Dluhy

Author Affiliations +

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166697
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

A method has been developed to determine the exact fractional composition in binary mixtures of phospholipids at the air-water (A/W) interface by ATR-IR spectroscopy in combination with ³¹P NMR spectroscopy and Langmuir-Blodgett surface chemistry. The procedure utilizes the wavenumber shift observed upon the synthetic replacement of hydrogen with deuterium in the lipid acyl chains to separate the vibrational bands due to each component in mixtures of deuterated and normally protiated lipids. The C-H and C-D integrated areas obtained from the IR spectra of the binary mixture are correlated with the exact mole fractions as determined using high resolution ³¹P NMR spectroscopy. We have used this technique to measure the fractional composition of binary mixtures of phospholipids at the A/W interface containing acyl chain perdeutereated phosphocholine lipids in combination with several protiated phosphoglycerol lipids.

Citation Download Citation

Amy D. Williams and Richard A. Dluhy "Combined IR and ³¹P NMR method for determining fractional composition in binary phospholipid monolayer films. Applications to phosphocholine: Phosphoglycerol mixtures", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166697

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