Cellulose antibody films for highly specific evanescent wave immunosensors

Andreas Hartmann; Daniel Bock; Thomas Jaworek; Sepp Kaul; Matthais Schulze; H. Tebbe; Gerhard Wegner; Stefan Seeger

doi:10.1117/12.229507

8 January 1996 Cellulose antibody films for highly specific evanescent wave immunosensors

Andreas Hartmann, Daniel Bock, Thomas Jaworek, Sepp Kaul, Matthais Schulze, H. Tebbe, Gerhard Wegner, Stefan Seeger

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229507
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

For the production of recognition elements for evanescent wave immunosensors optical waveguides have to be coated with ultrathin stable antibody films. In the present work non amphiphilic alkylated cellulose and copolyglutamate films are tested as monolayer matrices for the antibody immobilization using the Langmuir-Blodgett technique. These films are transferred onto optical waveguides and serve as excellent matrices for the immobilization of antibodies in high density and specificity. In addition to the multi-step immobilization of immunoglobulin G(IgG) on photochemically crosslinked and oxidized polymer films, the direct one-step transfer of mixed antibody-polymer films is performed. Both planar waveguides and optical fibers are suitable substrates for the immobilization. The activity and specificity of immobilized antibodies is controlled by the enzyme-linked immunosorbent assay (ELISA) technique. As a result reduced non-specific interactions between antigens and the substrate surface are observed if cinnamoylbutyether-cellulose is used as the film matrix for the antibody immobilization. Using the evanescent wave senor (EWS) technology immunosensor assays are performed in order to determine both the non-specific adsorption of different coated polymethylmethacrylat (PMMA) fibers and the long-term stability of the antibody films. Specificities of one-step transferred IgG-cellulose films are drastically enhanced compared to IgG-copolyglutamate films. Cellulose IgG films are used in enzymatic sandwich assays using mucine as a clinical relevant antigen that is recognized by the antibodies BM2 and BM7. A mucine calibration measurement is recorded. So far the observed detection limit for mucine is about 8 ng/ml.

Citation Download Citation

Andreas Hartmann, Daniel Bock, Thomas Jaworek, Sepp Kaul, Matthais Schulze, H. Tebbe, Gerhard Wegner, and Stefan Seeger "Cellulose antibody films for highly specific evanescent wave immunosensors", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229507

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available