Real-time optically sectioned wide-field microscopy employing structured light illumination and a CMOS detector

Jelena Mitic; Tiemo Anhut; Alexandre Serov; Theo Lasser; Stephane Bourquin

doi:10.1117/12.478024

9 July 2003 Real-time optically sectioned wide-field microscopy employing structured light illumination and a CMOS detector

Jelena Mitic, Tiemo Anhut, Alexandre Serov, Theo Lasser, Stephane Bourquin

Author Affiliations +

Proceedings Volume 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X; (2003) https://doi.org/10.1117/12.478024
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Real-time optically sectioned microscopy is demonstrated using an AC-sensitive detection concept realized with smart CMOS image sensor and structured light illumination by a continuously moving periodic pattern. We describe two different detection systems based on CMOS image sensors for the detection and on-chip processing of the sectioned images in real time. A region-of-interest is sampled at high frame rate. The demodulated signal delivered by the detector corresponds to the depth discriminated image of the sample. The measured FWHM of the axial response depends on the spatial frequency of the projected grid illumination and is in the μm-range. The effect of using broadband incoherent illumination is discussed. The performance of these systems is demonstrated by imaging technical as well as biological samples.

Citation Download Citation

Jelena Mitic, Tiemo Anhut, Alexandre Serov, Theo Lasser, and Stephane Bourquin "Real-time optically sectioned wide-field microscopy employing structured light illumination and a CMOS detector", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); https://doi.org/10.1117/12.478024

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